CRYAA monoclonal antibody, clone c9F2
产品名称： CRYAA monoclonal antibody, clone c9F2
英文名称： CRYAA monoclonal antibody, clone c9F2
产品编号： MAB1054
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant CRYAA.
- Immunogen:
- Recombinant protein corresponding to full length human CRYAA.
- Host:
- Mouse
- Reactivity:
- Human, Rat
- Form:
- Liquid
- Isotype:
- IgG1, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Small heat shock proteins from extremophiles: a review.
Laksanalamai P, Robb FT.Extremophiles. 2004 Feb;8(1):1-11. Epub 2003 Nov 19.
- 2.
- Characterization of alphaA-crystallin from high molecular weight aggregates in the normal human lens.
Fujii N, Awakura M, Takemoto L, Inomata M, Takata T, Fujii N, Saito T.Mol Vis. 2003 Jul 7;9:315-22.
- Applications
- Western Blot (Tissue lysate)
- Immunoblot analysis of rat eye extracts were resolved by electrophoresis , transferred to PVDF membrane and probed with CRYAA monoclonal antibody , clone c9F2 (1 : 1000) (Cat # MAB1054) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and a DAP detection system. Arrow indicates CRYAA (~ 20 kDa) .
- Entrez GeneID:
- 1409
- GeneBank Accession#:
- NM_000394
- Protein Accession#:
- NP_000385
- Gene Name:
- CRYAA
- Gene Alias:
- CRYA1,HSPB4
- Gene Description:
- crystallin, alpha A
- Omim ID:
- 123580
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq
- Other Designations:
- crystallin, alpha-1,human alphaA-crystallin (CRYA1)
- Related Disease