ARHGEF6 monoclonal antibody (M02), clone 2E5
产品名称： ARHGEF6 monoclonal antibody (M02), clone 2E5
英文名称： ARHGEF6 monoclonal antibody (M02), clone 2E5
产品编号： H00009459-M02
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant ARHGEF6.
- Immunogen:
- ARHGEF6 (AAH39856.1, 61 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- QTEADCINNINDFLKGCATLQVEIFDPDDLYSGVNFSKVLSTLLAVNKATEDQLSERPCGRSSSLSAANTSQTNPQGAVSSTVSGLQRQSKTVEMTENGSHQLIVKARFNFKQTN
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.06 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of ARHGEF6 expression in transfected 293T cell line by ARHGEF6 monoclonal antibody (M02), clone 2E5.
Lane 1: ARHGEF6 transfected lysate (Predicted MW: 87.5 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of ARHGEF6 transfected lysate using anti-ARHGEF6 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ARHGEF6 monoclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged ARHGEF6 is approximately 0.03ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between CDC42 and ARHGEF6 HeLa cells were stained with anti-CDC42 rabbit purified polyclonal 1:1200 and anti-ARHGEF6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Entrez GeneID:
- 9459
- GeneBank Accession#:
- BC039856
- Protein Accession#:
- AAH39856.1
- Gene Name:
- ARHGEF6
- Gene Alias:
- COOL2,Cool-2,KIAA0006,MRX46,PIXA,alpha-PIX,alphaPIX
- Gene Description:
- Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It may form a complex with G proteins and stimulate Rho-dependent signals. This protein is activated by PI3-kinase. Mutations in this gene can cause X-chromosomal non-specific mental retardation. [provided by RefSeq
- Other Designations:
- OTTHUMP00000062283,PAK-interacting exchange factor, alpha,Rac/Cdc42 guanine exchange factor (GEF) 6,Rac/Cdc42 guanine nucleotide exchange factor 6,mental retardation, X-linked 46,rho guanine nucleotide exchange factor 6
- Gene Pathway