Janus激酶3(JAK3)重组蛋白说明书-分析方法-资讯-生物在线

Janus激酶3(JAK3)重组蛋白说明书

作者:上海沪震实业有限公司 2023-04-16T00:00 (访问量:186)

 Janus激酶3(JAK3)重组蛋白说明书

Recombinant Janus Kinase 3 (JAK3) 

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Trp716~Asp967

Tags: N-terminal His-Tag

Subcellular Location: Endomembrane system; Peripheral membrane protein. Cytoplasm. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.5

Predicted Molecular Mass: 32.3kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

2022112403074568016

[IDENTIFICATION]

2022112403075306079

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用

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