钙蛋白酶6(CAPN6)重组蛋白说明书

Recombinant Calpain 6 (CAPN6)

[PROPERTIES]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu26~Arg495

Tags: N-terminal His-Tag

Tissue Specificity: Placenta. Subcellular Location: Cytoplasm, perinuclear region, cytoskeleton, spindle. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.9

Predicted Molecular Mass: 58.7kDa

Accurate Molecular Mass: 59kDa as determined by SDS-PAGE reducing conditions.

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37ºCfor 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[SEQUENCE]

[IDENTIFICATION]

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用