丝氨酸肽酶抑制因子Kazal型5(SPINK5)重组蛋白说明书

Recombinant Phospholipase C Beta 3, Phosphoinositide Specific (PLCb3)

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asp318~Lys468

Tags: N-terminal His Tag

Subcellular Location: Membrane

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation:100mMNaHCO₃, 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL,5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 20.6kDa

Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 100mMNaHCO₃, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Donot vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8ºC for one month.

 Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for in vitro and research use only, we will not be responsible for any issue ifthe kit was used in clinical diagnostic or any other procedures.
本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用