p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress. Four isoforms of p38 MAP kinase, a, b, g and d, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors. MKK3 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2.

Specificity/Sensitivity:

Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Antibody detects endogenous levels of p38 MAP kinase only when activated by dual phospho-rylation at threonine 180 and tyrosine 182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK.

Source/Purification:

Monoclonal antibodies are produced by immunizing mice with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase. Antibodies are purified by protein A and peptide affinity chromatography.

Storage and Stability:

Each vial contains 100 ul affinity purified mouse anti- Anti-Phospho-p38 MAP Kinase (Thr180/Tyr182) antibody in a special stabilizer. Real time studies that this special stabilizer helped retain most of antibody activity for 24 months while store at 4℃.

Recommended Antibody Dilutions: